PENETAPAN KADAR FENOLIK TOTAL DAN KADAR TANIN TOTAL EKSTRAK ETANOL 96% DAUN WIJAYA KUSUMA (Epiphyllum oxypetalum (DC.) Haw.) DENGAN METODE SPEKTROFOTOMETRI

Abstract

Abstrak Latar belakang: Indonesia memiliki berbagai macam tanaman hias, salah satunya merupakan spesies kaktus. Spesies kaktus yang paling banyak dibudidayakan adalah Epiphyllum oxypetalum (DC.) Haw. atau dikenal dengan nama Wijaya Kusuma. Berdasarkan uji fitokimia, ekstrak etanol daun Wijaya Kusuma dilaporkan mengandung senyawa golongan flavonoid, fenol, tanin dan saponin.  Tujuan: Ekstrak etanol 96% daun wijaya kusuma akan ditentukan kadar fenolik total dan kadar tannin total menggunakan metode spektrofotometri. Metode: Penentuan kadar fenolik total menggunakan metode Folin-Ciocalteu dan asam galat sebagai pembanding. Penentuan kadar tannin total dengan metode Vanilin-HCl dan katekin sebagai pembanding. Hasil: Hasil penapisan fitokimia dan identifikasi KLT mengandung senyawa fenol dan tannin. Kadar fenolik total ekstrak etanol daun E. oxypetalum sebesar 369,7 ± 57,675mg GAE/g ekstrak, sedangkan kadar tannin total sebesar 31,56 ± 1,285 %b/b EK. Simpulan: Ekstrak etanol 96% daun Wijaya Kusuma menunjukkan hasil positif mengandung fenolik dan tannin yang ditunjukkan dengan kadar total senyawa tersebut.Kata Kunci : Fenolik, Tanin, Spektrofotometri, Epiphyllum oxypetalum      
https://doi.org/10.56710/wiyata.v10i2.554
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ISSN Print: 2355-6498

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