Keywords
fibroblast viability
tooth avulsion
Abstract
Background: Avulsion is a complex trauma characterized by the loss of a tooth from its socket. An avulsion condition requires immediate replantation to return the tooth to its socket. The success of this action is influenced by the viability of the fibroblast cells. One of the measures to maintain viability is to store avulsed teeth in storage media. Avocado seed (Persea Americana Mill) is a plant that is easily available and contains flavonoids and saponins which have anti-inflammatory, antioxidant and antibacterial activities. Avocado seed extract is known to play a role in wound healing by increasing the number of fibroblast cells. Objective: to determine the effectiveness of avocado seed extract (Persea Americana Mill) in maintaining the viability of avulsed tooth fibroblast cells in male Wistar rats. Methods: The sample used was 24 Wistar rat teeth extracted and divided into 2 groups, 1 control group and 1 treatment group, where each group was divided into 2 subgroups based on soaking time, namely 30 and 60 minutes. Furthermore, fibroblast cells in the periodontal ligament are trypsinated and then counted with a hemocytometer on a light microscope. The data were then tested using SPSS 24 software. Results: There was a significant difference in the amount of fibroblast cell viability in immersion using avocado seed extract and HBSS. Conclusion: Soaking using HBSS for 30 minutes produced the best fibroblast cell viability.References
Abraham, Y., Christy, R., Gomez-Kunicki, A., Cheng, T., Eskarous, S., Samaan, V., Khan, A., & Sholapurkar, A. 2021. Management of dental avulsion injuries: A survey of dental support staff in cairns, australia. Dentistry Journal, 9(1), 1–13. https://doi.org/10.3390/dj9010004
Alim, N., Hasan, T., Rusman, & Jasmiadi. 2022. Uji Aktivitas Antioksidan Ekstrak Biji Alpukat (Persea americana Mill .) Asal Enrekang. Prosiding Seminar Nasional SAINS Dan Terapan (SINTA), VI(April), 166–175.
Ambarwati, R., & Rustiani, E. 2022. Formulasi dan Evaluasi Nanopartikel Ekstrak Biji Alpukat (Persea Americana Mill) Dengan Polimer Plga. Majalah Farmasetika, 7(4), 305. https://doi.org/10.24198/mfarmasetika.v7i4.38549
Arbi, T.A., Novita, C.F. & Mulya. 2016. Tingkat Pengetahuan Dokter Gigi Muda Terhadap Penanganan Trauma Dentoalveolar Di Rumah Sakit Gigi Mulut Universitas Syiah Kuala. Cakradonya Dent Journal, Vol. 8, No. 2:98-104
Aslantürk, Ö. S. 2018. In Vitro Cytotoxicity and Cell Viability Assays: Principles, Advantages, and Disadvantages. Genotoxicity - A Predictable Risk to Our Actual World, 1–18. https://doi.org/10.5772/intechopen.71923
Demir, P., Guler, C., Kizilci, E., & Keskin, G. 2020. Survival of Avulsed Permanent Incisors in Children Following Delayed Replantation. Niger J Clin Pract Vol. 23, Issue 5:631-637
Luthfi, M., Juliastuti, W.S., Rizky, Y.A., Wijayanti, E.H., Rachmawati, A.E. & Asyhari, N.P.O. 2020. Expression of Fibroblas Cells After Extraction of Wistar Rat Teeth After Topical Application of Okra Fruit (Abelmoschus esculentus) Gel. Infectious Disease Reports, Volume 12, Number s1: 8476.
Fajriyany, F. R., Harijadi, A., Kamadjaja, D. B., Program, M., Gigi, S. K., Departemen, S., Mulut, B., Fakultas, M., & Gigi, K. 2016. Perbandingan efektivitas air kelapa kemasan dan susu UHT sebagai media penyimpanan gigi avulsi (Comparison of effectiveness of bottled coconut water and UHT milk as storage media for avulsed tooth). J. Oral and Maxillofacial Surgery, 5(2), 20–26.
Fouad, A. F., Abbott, P. V., Tsilingaridis, G., Cohenca, N., Lauridsen, E., Bourguignon, C., O’Connell, A., Flores, M. T., Day, P. F., Hicks, L., Andreasen, J. O., Cehreli, Z. C., Harlamb, S., Kahler, B., Oginni, A., Semper, M., & Levin, L. 2020. International Association of Dental Traumatology guidelines for the management of traumatic dental injuries: 2. Avulsion of permanent teeth. Dental Traumatology, 36(4), 331–342. https://doi.org/10.1111/edt.12573
Kaur, B., Faktorová D, & Lukeš J. 2018. Cell Counting using a Haemocytometer (Naeubauer cell chamber) with Fixer. Genotoxicity - A Predictable Risk to Our Actual World , 1–17.
Kenny, J. D., Barret, E. J., Johnston, D. H., Sigal, M. J., & Tetenbaum, H. C. 2000. Clinical Management of Avulsed Permanent Incisors Using emdogain: Initial Report of an Investigation. Clinical Practice. 66(1):21.
Khinda, V. I., Brar, G. S., Kallar, S., & Khurana, H. 2017. Clinical and Practical Implications of Storage Media used for Tooth Avulsion. International Journal of Clinical Pediatric Dentistry, 10(2), 158–165. https://doi.org/10.5005/jp-journals-10005-1427
Kurnia, P. A., Ardhiyanto, H., & Suhartini. 2015. Potensi ekstrak teh hijau (Camellia sinensis) terhadap peningkatan jumlah sel fibroblas soket pasca pencabutan gigi pada tikus wistar. E-Jurnal Pustaka Kesehatan, 3(1), 122–127.
Moradian, H., Badakhsh, S., Rahimi, M., & Hekmatfar, S. 2013. Replantation of an avulsed maxillary incisor after 12 hours: Three-year follow-up. Iranian Endodontic Journal, 8(1), 33–36.
Mutiarahmi, C. N., Hartady, T., & Lesmana, R. (2021). Use of Mice As Experimental Animals in Laboratories That Refer To the Principles of Animal Welfare: a Literature Review. Indonesia Medicus Veterinus, 10(1), 134–145. https://doi.org/10.19087/imv.2020.10.1.134
Ulya, I., & Putra, I. P. R. A. (2016). Pengaruh Pemberian Gel Ekstrak Daun Melati (Jasminum Sambac L. Ait) Terhadap Jumlah Fibroblas Kulit Dalam Penyembuhan Luka Bakar Derajat Ii A Pada Tikus Putih (Rattus Norvegicus) Galur Wistar. Jurnal Ilmu Keperawatan (Journal of Nursing Science), 4(1), 1–11. https://doi.org/10.21776/ub.jik.2016.004.01.1
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